UCHL1 ELISA
Cat# ELISA-UCHL1

Ubiquitin C-terminal hydrolase 1 (UCHL1) has several other names, such as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). The protein is extremely abundant, and was estimated to be present at a concentration of 200-500 micrograms/g wet weight, representing a major protein component of neuronal cytoplasm (1). This has been claimed to represent 1-2% of total brain protein (2). It was later found that a ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to UCHL1. This is the first of a family of ubiquitin C-terminal hydrolases which have been characterized, many of which also have rigid cell type specific expression patterns.

The kit contains one standard ELISA 96 well plate coated with the mouse monoclonal antibody which has been blocked and so is ready to use. The plate can be separated into 8 12 well strips. The kit includes enough of the rabbit anti UCHL1 detection antibody and a pure recombinant full length human UCHL1 protein standard. Also included are a goat anti-rabbit alkaline phosphatase conjugate, p-Nitrophenol phosphatase substrate and buffer concentrates. We also include detailed instructions.